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KMID : 0382619960160010017
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Hanyang Journal of Medicine
1996 Volume.16 No. 1 p.17 ~ p.27
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Interrelationship between RNase Isozymes, RNase Inhibitors and Proteins in Cancer Tissue and Gastric Juice of Patients with Adenocarcinoma of Stomach
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Abstract
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Ribonuclease(RNase) known to be associated with carcinogenesis processes was determined in its activity in stomach cancer tissue and gastric juice of patients with stomach cancer. RNases, RNase inhibitors and proteins were isolated from the cancer tissue and gastric juice of patients with stomach cancer and substrate specificitiy of them were studied. The results were compared with those obtained from gastric juice and stomach tissue of normal control in order to find out whether cancer specific RNases were present and were released from the cancer tissue into the gastric juice.
RNase activity was significantly decrased and RNase inhibitor activity was unchanged in the stomach cancer tissue, while RNase and RNase inhibitor activities were markedly increased in gastirc juice of patients with stomach cancer. This indicates that RNase activities in the gastric juice could be used as biochemical and clinical markers for the stomach cancer.
DEAE-cellulose oclumn chromatography revealed that proteins and RNases in the stomach cancer tissue were separated into 7 protein peaks and 6 isozymes each, of which one RNase isozyme appeared to be specific to the cancer. Proteins and RNases in the gastric juice of the patients with stomach cancer were separated into 8 protein peaks and 4 isozymes each, of which 2 proteins and 4 isoyzmes were speicfic to the cancer.
Substrate specificity, ratio of RNase inhibitor/RNase and ratio of RNA/poly C for RNase activity of RNase isozymes isolated from the gastric juice of patients with stomach cancer were observed to be similar to those of RNase isozymes isolated from the stomach cancer tissue, indicating that the RNase isozymes in the gastric juice were released from the cancer tissue.
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